FastQCFastQC Report
Fri 8 Nov 2024
HJNLYAFX7_n02_jz7340_1_06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNLYAFX7_n02_jz7340_1_06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16614661
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC10158426.1141301649188025Illumina Single End PCR Primer 1 (100% over 50bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC2083941.2542777731065353Illumina Single End PCR Primer 1 (100% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1090560.6563841416926894No Hit
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC764220.45996725422203916Illumina Single End PCR Primer 1 (100% over 40bp)
GTGACTGGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT317920.19134907417009592Illumina Single End PCR Primer 1 (100% over 39bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATC250420.15072230483667406Illumina Single End PCR Primer 1 (97% over 38bp)
AGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCC236290.14221776778954442Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC182050.10957190158739923Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)