FastQCFastQC Report
Fri 8 Nov 2024
HJNLYAFX7_n02_jz7340_1_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNLYAFX7_n02_jz7340_1_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18262265
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC6522213.571413513055473Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2327491.2744804655939448No Hit
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1342750.735259290126389Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC581970.31867350517583665Illumina Single End PCR Primer 1 (100% over 40bp)
GTGACTGGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT232240.1271693297627649Illumina Single End PCR Primer 1 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)