FastQCFastQC Report
Fri 8 Nov 2024
HJNLYAFX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNLYAFX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11227269
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA10565059.410169115926589TruSeq Adapter, Index 14 (97% over 38bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAT1059460.9436488962721032TruSeq Adapter, Index 2 (97% over 37bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCT908310.809021321213556TruSeq Adapter, Index 14 (97% over 34bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTG732210.6521710667126618TruSeq Adapter, Index 14 (96% over 33bp)
GAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAAC470920.4194430542280585TruSeq Adapter, Index 14 (96% over 30bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCG463090.41246896284394713TruSeq Adapter, Index 14 (97% over 38bp)
AAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGA327040.29129078496293265TruSeq Adapter, Index 14 (96% over 32bp)
AGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAA293790.2616753905157167TruSeq Adapter, Index 14 (96% over 31bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC272050.24231182133428886TruSeq Adapter, Index 14 (97% over 36bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATC257150.2290405618677169TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACAGATCGGAAGAGCACAC224570.20002192875221927Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACT218040.1942057324893525TruSeq Adapter, Index 14 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCGGAAGAGCAC173550.1545789986861453TruSeq Adapter, Index 7 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTC170120.15152393694316935TruSeq Adapter, Index 14 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAGATCGGAAGAGCA131880.11746400660748399TruSeq Adapter, Index 3 (97% over 37bp)
AGATGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC113970.10151177459095352TruSeq Adapter, Index 14 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)