FastQCFastQC Report
Fri 8 Nov 2024
HJNLYAFX7_n01_jz7340_1_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNLYAFX7_n01_jz7340_1_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20469789
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTG2351861.1489419846975464TruSeq Adapter, Index 10 (97% over 42bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC1611580.7872968304656194TruSeq Adapter, Index 10 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTG1285970.6282282636132692TruSeq Adapter, Index 10 (97% over 42bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT633160.3093143754437332TruSeq Adapter, Index 10 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGATG541740.2646534363397688TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGGG527240.2575698264403214TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTT426230.20822393430630867TruSeq Adapter, Index 10 (97% over 42bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT366930.17925441244167195TruSeq Adapter, Index 10 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTT342540.16733929206598075TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTTTG240880.11767585879854453TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTGTG211720.10343047502834543TruSeq Adapter, Index 10 (97% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)