FastQCFastQC Report
Fri 8 Nov 2024
HJNLYAFX7_n01_jz7340_1_06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNLYAFX7_n01_jz7340_1_06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16614661
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTG3865332.326457337889711TruSeq Adapter, Index 8 (97% over 42bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT3200061.926045918120147TruSeq Adapter, Index 8 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTG1716121.032894983532917TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGGG1404410.845283572141496TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA1274850.7673042501438939TruSeq Adapter, Index 14 (97% over 38bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA1184080.712671778256565TruSeq Adapter, Index 8 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTT765090.4604908881378922TruSeq Adapter, Index 8 (97% over 42bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG676420.40712236018538084TruSeq Adapter, Index 8 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTT553740.33328395926946686TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGATG516100.31062926893302245TruSeq Adapter, Index 8 (97% over 42bp)
TCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA266670.16050282337990524TruSeq Adapter, Index 8 (100% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGATT192710.11598792175175887TruSeq Adapter, Index 8 (97% over 42bp)
CACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGT188190.11326743290158012Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGTGTG182090.10959597670996718TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGTTTG177150.1066226990728249TruSeq Adapter, Index 8 (97% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)