FastQCFastQC Report
Fri 8 Nov 2024
HJNLYAFX7_n01_jz7340_1_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNLYAFX7_n01_jz7340_1_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18984328
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG4758802.5066992100010075TruSeq Adapter, Index 6 (100% over 49bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA2338941.2320372888626872TruSeq Adapter, Index 6 (100% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT889780.468691859938366TruSeq Adapter, Index 6 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATG592980.3123523782353529TruSeq Adapter, Index 6 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTTTG535190.28191147982693937TruSeq Adapter, Index 6 (97% over 49bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA517510.27259853495999437TruSeq Adapter, Index 6 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGGATG299720.15787759250682984TruSeq Adapter, Index 6 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATT297200.15655018181312502TruSeq Adapter, Index 6 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA231510.12194795623000193TruSeq Adapter, Index 14 (97% over 38bp)
TCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA219760.11575864049546553TruSeq Adapter, Index 6 (100% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTGTG195270.10285852625386582TruSeq Adapter, Index 6 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)