FastQCFastQC Report
Fri 8 Nov 2024
HJNLYAFX7_n01_jz7340_1_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNLYAFX7_n01_jz7340_1_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13716849
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG703650.5129822454121935TruSeq Adapter, Index 5 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGGGGGTG446850.3257672370673469TruSeq Adapter, Index 5 (97% over 41bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG294050.21437139098053787TruSeq Adapter, Index 5 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGGGGTTG223590.16300390855071745TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGGATG201920.1472058196456052TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGGGGATG195040.14219009045007347TruSeq Adapter, Index 5 (97% over 41bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGT178200.12991321840752204TruSeq Adapter, Index 5 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATG163420.11913814900200476TruSeq Adapter, Index 5 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)