FastQCFastQC Report
Fri 8 Nov 2024
HJNLYAFX7_n01_jz7340_1_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNLYAFX7_n01_jz7340_1_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14246224
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT1037480.7282491135896783TruSeq Adapter, Index 2 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGGTG791640.555684088639909TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA730680.5128938025963933TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGTTG513610.3605236026051535TruSeq Adapter, Index 2 (97% over 42bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT441430.30985754540992755TruSeq Adapter, Index 2 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGATG305220.21424624518047727TruSeq Adapter, Index 2 (97% over 42bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG244130.1713647068865406TruSeq Adapter, Index 2 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGGTT156550.10988876771837927TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGTATG149780.1051366312926148TruSeq Adapter, Index 2 (97% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)