FastQCFastQC Report
Fri 8 Nov 2024
HJNLYAFX7_n01_jz7340_1_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNLYAFX7_n01_jz7340_1_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16777575
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG3186141.8990467931152148TruSeq Adapter, Index 1 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA1352850.8063441826366445TruSeq Adapter, Index 14 (97% over 38bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA1052240.6271704939480229TruSeq Adapter, Index 1 (100% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG404890.24132808227649108TruSeq Adapter, Index 1 (100% over 39bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC249320.1486031205344038TruSeq Adapter, Index 1 (100% over 38bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCT207460.12365315011257587TruSeq Adapter, Index 14 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)