FastQCFastQC Report
Mon 6 Mar 2017
HJNLLAFXX_n02_lbstarc-1_fem.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNLLAFXX_n02_lbstarc-1_fem.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12205558
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGCTA3188602.612416408983514No Hit
TCTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA1834991.503405251935225No Hit
ATCCAGAGTCACCAAAAGGACGGGGTTGCCCCCGATTGGTTTTGGTCTAATAAAAGCGCTCTTTCCCGTAAGGGT1411091.156104456674574No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG1036370.8490967803356471Illumina Single End PCR Primer 1 (100% over 58bp)
GTGCTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATA878770.7199752768369951No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG525260.4303449297443017No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCAG493100.40399627776132807No Hit
GTTGCCTCCCAACGCCGAGGCGCAAACGGTACCGTTACACTTTTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC491240.40247238184440237No Hit
CTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCAGCCGGGCTCACGC488190.3999735202601962No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGCTAC404290.3312343442225255No Hit
ACTGTGTTGGATCGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTAGTC403770.33080830880489037No Hit
CCAGAGTCACCAAAAGGACGGGGTTGCCCCCGATTGGTTTTGGTCTAATAAAAGCGCTCTTTCCCGTAAGGGTCG390860.3202311602632178No Hit
CTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATATGC365980.29984700412713616No Hit
TCCATGCACCATTATTCAGGCTGAAGAGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCCCGGCCACC361530.29620112411083543No Hit
GTTGGATCGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTAGTCTTTCG339870.2784551103685714No Hit
CCAACGTGTACGCTCTTGCTCCGCCTCACGGACGCGGCCGACGAGACGGGCCGGCGATGCGCGCAGCTCGGTACG330720.27095852561595296No Hit
CAAAAGGACGGGGTTGCCCCCGATTGGTTTTGGTCTAATAAAAGCGCTCTTTCCCGTAAGGGTCGGAGCTTGCTC322880.26453522239622307No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGCTACCC315530.25851337562772636No Hit
GGGGTGGGTATCGCTTCGGCACGGAGCCCGCCCTGCTCGGCGGACCCCTGAATGCCGTCAGTTGCTGTGGCTCGT311220.254982197454635No Hit
CTCCTCGTTCGTTTGCGTTGCCGCACTCGCCCCCGAGGGGACAATCTCCGCGTCCGGGTTCGGGAATATTAACCC307850.2522211602288072No Hit
TGGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTGATGACTCGCGCTTACTGGGAATTCCTCGTTCATGG271180.22217747029672874No Hit
TGCTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATAT245290.2009658222917789No Hit
GCTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATATG243800.19974506696047817No Hit
CTGTGTTGGATCGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTAGTCT235090.19260897371508948No Hit
GGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTGATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGG203810.1669813047465753No Hit
CCCAGTTCTCACCGACCGACCAGACCGACAAACGGCGGCGGCGGCGTTCGGTAATGATCCTTCCGCAGGTTCACC183690.1504970112796154No Hit
CTCTGACTTTCGTTCTTGATTAATGAAAACGTTCTTGGCAAACGCTTTCGCAGCCGGTCGTCTTGCGGCGATCCA163780.13418477057746972No Hit
AAAAGGACGGGGTTGCCCCCGATTGGTTTTGGTCTAATAAAAGCGCTCTTTCCCGTAAGGGTCGGAGCTTGCTCG156490.12821208174177698No Hit
CCCAACGTGTACGCTCTTGCTCCGCCTCACGGACGCGGCCGACGAGACGGGCCGGCGATGCGCGCAGCTCGGTAC155560.12745013378331413No Hit
GTCGTCTACGAATGATTTGGCACCTCTACATTGCATGGGGTGGGTATCGCTTCGGCACGGAGCCCGCCCTGCTCG153890.12608190465360125No Hit
GTGGGTATCGCTTCGGCACGGAGCCCGCCCTGCTCGGCGGACCCCTGAATGCCGTCAGTTGCTGTGGCTCGTCAC152150.12465632460228364No Hit
TTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCAGCCGGGCTCACGCC137150.11236684140126982No Hit
GGTGGGTATCGCTTCGGCACGGAGCCCGCCCTGCTCGGCGGACCCCTGAATGCCGTCAGTTGCTGTGGCTCGTCA131030.10735273225525618No Hit
CGACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGTCCGGCGCGAAAATCACACACTCTCCC130450.10687753890481697No Hit
CGTCGTCTACGAATGATTTGGCACCTCTACATTGCATGGGGTGGGTATCGCTTCGGCACGGAGCCCGCCCTGCTC122120.10005277923385396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCTGC461800.063.8740121
CCGTATC165950.061.6580249
GCCGTAT167800.060.95842748
CGTATCA170550.060.11612350
CGCCGTA170450.060.010747
TGGTCGC185050.055.0504843
GGTCGCC187600.054.44930644
ATCCAGA222700.054.3965261
TCTCGGT188600.054.0667137
TCCAGAG225200.054.0130582
GTATCAT191050.053.6116151
ATCTCGG192450.053.0922136
TCGGTGG195500.052.38871839
CGGTGGT195900.052.10565640
GTGGTCG196150.051.9873342
CTCGGTG197550.051.8448638
TCTCCAC291800.051.1119081
GTCGCCG204150.050.1366145
GCCTGCT596100.049.405622
CCCGCTA521500.049.38801269
Produced by FastQC (version 0.11.2)