FastQCFastQC Report
Mon 6 Mar 2017
HJNLLAFXX_n02_lbstara_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNLLAFXX_n02_lbstara_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12205066
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG424180.34754420828203636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC33150.024.3519949
CGCCGTA35150.023.0644447
GCCGTAT35150.022.96648248
CGTATCA42100.019.09307950
GGTCGCC45000.018.47488644
TCGCCGT44400.018.33652146
TCTCGGT43700.018.31334937
ATCTCGG44050.018.24600236
CTCGGTG49450.016.4629938
GTCGCCG50300.016.39146645
GATCTCG50450.016.13653635
TCGGTGG50200.016.07959739
TGGTCGC51650.015.49511843
CGGTGGT54950.014.94074540
GTGGTCG56850.014.32034242
AGATCTC62350.013.33326334
GTAGATC82500.010.24392132
GTATCAT83150.09.99896851
AGTGTAG85500.09.48103729
GTGTAGA91900.09.27119230
Produced by FastQC (version 0.11.2)