FastQCFastQC Report
Mon 6 Mar 2017
HJNLLAFXX_n02_lbstara_fem.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNLLAFXX_n02_lbstara_fem.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555886
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG182483.2826874575002787Illumina Single End PCR Primer 1 (100% over 58bp)
TCTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA152772.748225355558514No Hit
TGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGCTA150822.71314622062797No Hit
ATCCAGAGTCACCAAAAGGACGGGGTTGCCCCCGATTGGTTTTGGTCTAATAAAAGCGCTCTTTCCCGTAAGGGT85511.5382650399542352No Hit
GTGCTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATA70751.2727429724799688No Hit
CTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCAGCCGGGCTCACGC61901.1135376677951954No Hit
CCCAGTTCTCACCGACCGACCAGACCGACAAACGGCGGCGGCGGCGTTCGGTAATGATCCTTCCGCAGGTTCACC51950.9345441331496026No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCAG50160.9023432862133604No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAGGGGGG44480.8001640624156752Illumina Single End PCR Primer 1 (100% over 58bp)
CCAGAGTCACCAAAAGGACGGGGTTGCCCCCGATTGGTTTTGGTCTAATAAAAGCGCTCTTTCCCGTAAGGGTCG36170.6506729797116675No Hit
GTTGCCTCCCAACGCCGAGGCGCAAACGGTACCGTTACACTTTTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC34300.6170329887782747No Hit
TCCATGCACCATTATTCAGGCTGAAGAGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCCCGGCCACC30470.5481339699146948No Hit
CTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATATGC28040.5044199710012485No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26830.48265291804434723No Hit
GCTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATATG23600.42454747915939595No Hit
CAAAAGGACGGGGTTGCCCCCGATTGGTTTTGGTCTAATAAAAGCGCTCTTTCCCGTAAGGGTCGGAGCTTGCTC22490.4045793561989329No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGCTAC19390.3488125263093512No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGCTACCC19120.3439554153189683No Hit
GGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTGATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGG18960.34107712732466733No Hit
TGCTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATAT18720.3367596953332158No Hit
GTCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTGATGACTCGCGCTTACTGGGAATTCCTCGTTCAT17910.32218836236206705No Hit
CGACCGACCAGACCGACAAACGGCGGCGGCGGCGTTCGGTAATGATCCTTCCGCAGGTTCACCTACGGAAACCTT17220.309775745386644No Hit
TTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCAGCCGGGCTCACGCC16670.29988163040623433No Hit
GTTGGATCGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTAGTCTTTCG14930.2685802484682111No Hit
ACTGTGTTGGATCGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTAGTC13660.245733837513447No Hit
TGGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTGATGACTCGCGCTTACTGGGAATTCCTCGTTCATGG13160.23673918753125642No Hit
AAAAGGACGGGGTTGCCCCCGATTGGTTTTGGTCTAATAAAAGCGCTCTTTCCCGTAAGGGTCGGAGCTTGCTCG11870.21353299057720468No Hit
CCAGTTCTCACCGACCGACCAGACCGACAAACGGCGGCGGCGGCGTTCGGTAATGATCCTTCCGCAGGTTCACCT11200.20148015960106927No Hit
GTCCCAGTTCTCACCGACCGACCAGACCGACAAACGGCGGCGGCGGCGTTCGGTAATGATCCTTCCGCAGGTTCA11040.1986018716067683No Hit
CTCCTCGTTCGTTTGCGTTGCCGCACTCGCCCCCGAGGGGACAATCTCCGCGTCCGGGTTCGGGAATATTAACCC10380.1867289336302767No Hit
GTACAAAGGGCAGGGACGTAATCAACGCGAGCTGATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGGGAAGA10300.1852897896331262No Hit
GTACGCTCTTGCTCCGCCTCACGGACGCGGCCGACGAGACGGGCCGGCGATGCGCGCAGCTCGGTACGCCGCGAT9540.17161792166019652No Hit
CCAACGTGTACGCTCTTGCTCCGCCTCACGGACGCGGCCGACGAGACGGGCCGGCGATGCGCGCAGCTCGGTACG9220.16586134567159455No Hit
GGGGTGGGTATCGCTTCGGCACGGAGCCCGCCCTGCTCGGCGGACCCCTGAATGCCGTCAGTTGCTGTGGCTCGT9130.16424230867480022No Hit
CTGTGTTGGATCGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTAGTCT8940.16082434168156778No Hit
ACTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA7870.14157579071967993No Hit
GCCGGTCGTCTTGCGGCGATCCAAGAATTTCACCTCTAACACCGCAATACGAATGCCCCCGTCCGTCCCTCTTAA6920.1244859557535178No Hit
TCTCCTTAGTCGCCCCAACTAAACTGTACTTTATAATCATTAATTAAAAAGATAAAGTAAAGCGCCATAGGGTCT6920.1244859557535178No Hit
CGACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGTCCGGCGCGAAAATCACACACTCTCCC6770.12178756075886063No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCCAAAAGCTTTGGTTTCCCGGAAGGTGCCCGACGGGTCATAGCAAA6090.10955483678308142No Hit
CCAAAAGGACGGGGTTGCCCCCGATTGGTTTTGGTCTAATAAAAGCGCTCTTTCCCGTAAGGGTCGGAGCTTGCT6090.10955483678308142No Hit
TGTTCGCCTATCTCTTAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTCTCCACTTCAGTCT6070.1091950507837938No Hit
AGAGTCACCAAAAGGACGGGGTTGCCCCCGATTGGTTTTGGTCTAATAAAAGCGCTCTTTCCCGTAAGGGTCGGA6020.10829558578557474No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTGATGACTCGCGCTTACTGGGAATTCCTCGTTCATG5910.10631676278949281No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCACTTTGGGCTGCATTCCCAAACAACCCGACTCCGGGGAGGC5910.10631676278949281No Hit
GTCGTCTACGAATGATTTGGCACCTCTACATTGCATGGGGTGGGTATCGCTTCGGCACGGAGCCCGCCCTGCTCG5750.10343847479519182No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTGATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGGG5680.10217922379768514No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT26350.067.1610348
CCGTATC26350.066.8991949
CGCCGTA26600.066.52981647
CGTATCA26550.066.3952450
GTATCAT26950.065.28178451
GGTCGCC27450.064.46969644
TGGTCGC27400.064.3355443
TCTCGGT27400.063.8261937
TCGGTGG27800.063.16166739
AGATCTC27950.063.06387334
GTCGCCG28300.062.77712245
ATCTCGG28000.062.70487236
TATCATT28100.062.6101152
CTCGGTG28000.062.46410838
GTGGTCG28250.062.15555242
TGCCTGC19550.061.971081
CATTAAA28700.061.5415855
CGGTGGT28600.061.39490540
TAGATCT28950.060.88549833
GTGTAGA29400.060.07090430
Produced by FastQC (version 0.11.2)