FastQCFastQC Report
Mon 6 Mar 2017
HJNLLAFXX_n02_lbhearte_fem.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNLLAFXX_n02_lbhearte_fem.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5111223
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG1425962.7898606654415197Illumina Single End PCR Primer 1 (100% over 58bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG323230.6323926778385525No Hit
TCTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA178340.3489184486765692No Hit
GTGCTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATA175630.3436163908324877No Hit
GTGCTGGGCTCTTCCCGCTTCACTCGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT98460.19263491340526523Illumina Single End PCR Primer 1 (100% over 46bp)
GTGCTGGGCTCTTCCCGCTTCACTCGCCGTTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG96580.1889567330558655Illumina Single End PCR Primer 1 (100% over 42bp)
CTCTGACTTTCGTTCTTGATTAATGAAAACGTTCTTGGCAAACGCTTTCGCAGCCGGTCGTCTTGCGGCGATCCA77570.15176406899092448No Hit
CTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATATGC68740.13448836022220123No Hit
GTGCTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT59800.1169974387734599Illumina Single End PCR Primer 1 (100% over 39bp)
CTCCTCGTTCGTTTGCGTTGCCGCACTCGCCCCCGAGGGGACAATCTCCGCGTCCGGGTTCGGGAATATTAACCC52370.10246080047769388No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA216650.064.60445450
CCGTATC216400.064.59811449
GCCGTAT217700.064.37083448
CGCCGTA220800.063.52957547
GTATCAT227400.061.64019851
GTCGCCG231950.060.54528845
GGTCGCC233100.060.18739344
TGGTCGC237900.058.9295243
TCATTAA240700.058.5203254
ATCATTA245250.057.3648553
CATTAAA247400.057.13071455
TATCATT246200.057.0173852
GTGGTCG253150.055.5027342
GGTGGTC277600.050.6510441
ATTAAAA282900.050.5093856
CGGTGGT289550.048.5487140
GTGCTGG108600.048.201531
TCGGTGG299150.047.0599339
AAAGGGG228750.046.53529466
AAGGGGG228050.046.5121567
Produced by FastQC (version 0.11.2)