FastQCFastQC Report
Mon 6 Mar 2017
HJNLLAFXX_n02_bay54c_fem.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNLLAFXX_n02_bay54c_fem.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10652264
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG1159361.0883695710132606Illumina Single End PCR Primer 1 (100% over 58bp)
GTGCTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATA1032420.9692024155616121No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG976630.9168285727803969No Hit
TCTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA527730.4954158101977195No Hit
CTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATATGC320240.30063092690905896No Hit
GGGGTGGGTATCGCTTCGGCACGGAGCCCGCCCTGCTCGGCGGACCCCTGAATGCCGTCAGTTGCTGTGGCTCGT303040.28448412468936185No Hit
CTCCTCGTTCGTTTGCGTTGCCGCACTCGCCCCCGAGGGGACAATCTCCGCGTCCGGGTTCGGGAATATTAACCC294940.2768801073649696No Hit
TGCTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATAT294770.2767205168779144No Hit
TGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGCTA240180.2254731951817942No Hit
GCTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATATG231420.21724959126059964No Hit
CTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCAGCCGGGCTCACGC157840.1481750733928487No Hit
GTGGGTATCGCTTCGGCACGGAGCCCGCCCTGCTCGGCGGACCCCTGAATGCCGTCAGTTGCTGTGGCTCGTCAC154230.14478612246185413No Hit
GGTGGGTATCGCTTCGGCACGGAGCCCGCCCTGCTCGGCGGACCCCTGAATGCCGTCAGTTGCTGTGGCTCGTCA142200.13349274858377524No Hit
CTCCGCCTCACGGACGCGGCCGACGAGACGGGCCGGCGATGCGCGCAGCTCGGTACGCCGCGATCTCGCCTAAGG125370.11769329036531576No Hit
GGGTGGGTATCGCTTCGGCACGGAGCCCGCCCTGCTCGGCGGACCCCTGAATGCCGTCAGTTGCTGTGGCTCGTC115200.10814602416913438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC183350.062.37309649
GCCGTAT185350.061.7369948
CGCCGTA186600.061.36012347
CGTATCA188550.060.72552550
GGTCGCC200450.057.15355344
TGGTCGC201500.056.8380743
GTCGCCG202050.056.80393245
GTGGTCG214000.053.55058742
TCTCGGT216150.052.714237
CGGTGGT218850.052.3953640
CTCGGTG218100.052.24362638
TCGGTGG218950.052.22962639
GTATCAT220300.051.9418651
ATCTCGG221100.051.59619536
GTGCTGG188500.048.905521
AGATCTC237650.048.17563234
GGTGGTC247750.046.3672141
TCATTAA248550.046.34375454
GATCTCG248550.045.8558235
CATTAAA259500.044.6139955
Produced by FastQC (version 0.11.2)