FastQCFastQC Report
Mon 6 Mar 2017
HJNLLAFXX_n02_bay46d-fem.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNLLAFXX_n02_bay46d-fem.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8526273
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA4934325.78719447524141No Hit
GTGCTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATA3872934.542348104500055No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGG2837713.328195097670459Illumina Single End PCR Primer 1 (100% over 58bp)
CTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATATGC1944362.2804336666208083No Hit
CTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCAGCCGGGCTCACGC1762382.066999262162964No Hit
CTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCAG1451951.7029128670874132No Hit
GCTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATATG1114731.3074059439569903No Hit
TGCTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATAT1085011.272548978903209No Hit
TGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGCTA912681.0704325324793142No Hit
CTCCTCGTTCGTTTGCGTTGCCGCACTCGCCCCCGAGGGGACAATCTCCGCGTCCGGGTTCGGGAATATTAACCC853091.0005426755629334No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAGGGGGG649540.7618099959970787Illumina Single End PCR Primer 1 (100% over 58bp)
GTTGCCTCCCAACGCCGAGGCGCAAACGGTACCGTTACACTTTTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC639260.7497531453660937No Hit
ACTGTGTTGGATCGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTAGTC593810.696447322294278No Hit
GTTGGATCGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTAGTCTTTCG575750.675265734512606No Hit
TTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCAGCCGGGCTCACGCC563350.6607224516503284No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG537190.6300408161924911No Hit
GTCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTGATGACTCGCGCTTACTGGGAATTCCTCGTTCAT488100.5724658358933616No Hit
GGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTGATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGG410120.4810073522159096No Hit
CTGTGTTGGATCGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTAGTCT348960.4092761280339018No Hit
CCAACGTGTACGCTCTTGCTCCGCCTCACGGACGCGGCCGACGAGACGGGCCGGCGATGCGCGCAGCTCGGTACG327230.3837901976631525No Hit
TCCATGCACCATTATTCAGGCTGAAGAGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGTCCCGGCCACC320940.37641300014672296No Hit
TGGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTGATGACTCGCGCTTACTGGGAATTCCTCGTTCATGG299090.3507863283289193No Hit
ATCCAGAGTCACCAAAAGGACGGGGTTGCCCCCGATTGGTTTTGGTCTAATAAAAGCGCTCTTTCCCGTAAGGGT262180.30749660490580116No Hit
GTCGTCTACGAATGATTTGGCACCTCTACATTGCATGGGGTGGGTATCGCTTCGGCACGGAGCCCGCCCTGCTCG262110.3074145057283528No Hit
CTCTGACTTTCGTTCTTGATTAATGAAAACGTTCTTGGCAAACGCTTTCGCAGCCGGTCGTCTTGCGGCGATCCA252830.29653050048948704No Hit
CGTCGTCTACGAATGATTTGGCACCTCTACATTGCATGGGGTGGGTATCGCTTCGGCACGGAGCCCGCCCTGCTC232860.27310877800886746No Hit
TGCTCTACTAAGTACGACACCCCGACTCCAACCTAGGTCGTCTACGAATGATTTGGCACCTCTACATTGCATGGG220060.25809635698974215No Hit
CGACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGTCCGGCGCGAAAATCACACACTCTCCC211520.24808025734104458No Hit
GTACAAAGGGCAGGGACGTAATCAACGCGAGCTGATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGGGAAGA208740.24481974715095328No Hit
ACTCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA182470.21400909869998297No Hit
TGTTCGCCTATCTCTTAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTCTCCACTTCAGTCT180670.2118979769941685No Hit
TGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTAGTGATATGCT171990.2017176789905742No Hit
GTACGCTCTTGCTCCGCCTCACGGACGCGGCCGACGAGACGGGCCGGCGATGCGCGCAGCTCGGTACGCCGCGAT162830.1909744151987627No Hit
CCCAACGTGTACGCTCTTGCTCCGCCTCACGGACGCGGCCGACGAGACGGGCCGGCGATGCGCGCAGCTCGGTAC159360.18690464168810922No Hit
CGCATGGGGTGGGTATCGCTTCGGCACGGAGCCCGCCCTGCTCGGCGGACCCCTGAATGCCGTCAGTTGCTGTGG159210.18672871487929132No Hit
GGGGTGGGTATCGCTTCGGCACGGAGCCCGCCCTGCTCGGCGGACCCCTGAATGCCGTCAGTTGCTGTGGCTCGT156660.18373795912938748No Hit
ATCGACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGTCCGGCGCGAAAATCACACACTCTC141570.16603972216230936No Hit
TCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCAGCCGGGCTCACGCCC135150.15851005474490437No Hit
TGAGCGTCGGCCGAAGCGGACACCCGCCCGAGGGCGGGGCCGCGCAGCTGAGGCAGTCCACCGGAAGGTCCGGCC134010.15717301099788852No Hit
TCTGCCGCGACGGAACGGTATAGGCCCGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGA130100.15258718551470263No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCCAAAAGCTTTGGTTTCCCGGAAGGTGCCCGACGGGTCATAGCAAA119620.14029576580529385No Hit
CAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCAGCCGGGCTCACGCCCA116300.13640191910345822No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTGATGACTCGCGCTTACTGGGAATTCCTCGTTCATGGGG110950.13012719625562072No Hit
CCAGTGCACCGGACTCCGCGGGAAGCGCGGAGCTTTACGGCGACTACGTCCCTATCTCCGGCCAAGCCGATTCCA109330.1282271867203877No Hit
CCAGAGTCACCAAAAGGACGGGGTTGCCCCCGATTGGTTTTGGTCTAATAAAAGCGCTCTTTCCCGTAAGGGTCG109190.12806298836549102No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGCTAC107360.12591668129791292No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCATGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGCTACCC106170.12452099528129114No Hit
TCTCCTTAGTCGCCCCAACTAAACTGTACTTTATAATCATTAATTAAAAAGATAAAGTAAAGCGCCATAGGGTCT105810.12409877094012824No Hit
CCCAGTTCTCACCGACCGACCAGACCGACAAACGGCGGCGGCGGCGTTCGGTAATGATCCTTCCGCAGGTTCACC103700.12162406716275681No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCACTTTGGGCTGCATTCCCAAACAACCCGACTCCGGGGAGGC101010.11846911305795627No Hit
GCCGGTCGTCTTGCGGCGATCCAAGAATTTCACCTCTAACACCGCAATACGAATGCCCCCGTCCGTCCCTCTTAA100120.11742528065897022No Hit
TCCACTTCAGTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCAGC100040.11733145302760069No Hit
GTTGAGCGTCGGCCGAAGCGGACACCCGCCCGAGGGCGGGGCCGCGCAGCTGAGGCAGTCCACCGGAAGGTCCGG97660.1145400809943571No Hit
TGCCTCCCAACGCCGAGGCGCAAACGGTACCGTTACACTTTTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT97480.11432896882377563No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTGATGACTCGCGCTTACTGGGAATTCCTCGTTCATG97010.11377773148947964No Hit
GTGGGTATCGCTTCGGCACGGAGCCCGCCCTGCTCGGCGGACCCCTGAATGCCGTCAGTTGCTGTGGCTCGTCAC95890.11246414465030617No Hit
GTGCTGGGCTCTTCCCGCTTCACTCGCCGTTACTAGGGGAATCCTTGTTAGTTTCTTTTCCAGATCGGAAGAGCG93080.10916844909845133No Hit
CCCAGTTCTCACCGACCGACCAGACCGACAAACGGCGGCGGCGGCGGCGTTCGGTAATGATCCTTCCGCAGGTTC92800.10884005238865797No Hit
CGTTGAGCGTCGGCCGAAGCGGACACCCGCCCGAGGGCGGGGCCGCGCAGCTGAGGCAGTCCACCGGAAGGTCCG92230.10817153051515005No Hit
GTCCCAGTCAAACTCCCCGCCTGACGCTGTCCTCAGAGCGGATCGCGGCGGCGCGAGCGCCGCCGCTTAACTCCA90440.10607213726325675No Hit
GTCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCAGCCGGGCTCAC90260.10586102509267531No Hit
TGCCTGAGGGTTGTTGGGCCATGCGCACCACGGAGAAGTCATTGGGGGTGAAGCCGGAGCCCTGGTCATAGTTGG89620.10511040404171904No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAAA411150.065.9399655
GTATCAT409550.065.7706951
CGTATCA410550.065.7201150
CCGTATC413000.065.2045649
GCCGTAT414150.065.16511548
TCATTAA421700.064.1515954
CGCCGTA421200.064.0498147
TATCATT422000.063.99191752
GTGCTGG565000.062.8092461
GGTCGCC434550.062.15101244
TGGTCGC436900.061.7689843
TCTCCAC669950.061.424921
GTCGCCG441600.061.15950445
TGCCTGC144750.060.9919131
ATTAAAA449150.060.82895356
ATCATTA447400.060.4591853
ACTGTGT85050.059.526011
TTAAAAA463150.059.02002357
GTGGTCG458350.058.9525242
GGTGGTC465200.057.9880541
Produced by FastQC (version 0.11.2)