FastQCFastQC Report
Mon 6 Mar 2017
HJNLLAFXX_n01_lbstarc-1_fem.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNLLAFXX_n01_lbstarc-1_fem.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12205558
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1303181.0676939145264805TruSeq Adapter, Index 7 (97% over 37bp)
GTTAGTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAA496150.40649513934553416No Hit
CTTTCGACGGTTAGTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGA483940.3964915000199089No Hit
CGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGC350010.28676280101245677No Hit
GACGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACG329600.27004091087027726No Hit
TTTCGACGGTTAGTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGAG322110.26390436225857106No Hit
GTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGG292000.23923527297973596No Hit
CCTATCGAAAGGGAATCGGGTTAATATTCCCGAACCCGGACGCGGAGATTGTCCCCTCGGGGGCGAGTGCGGCAA281540.23066540669422897No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGACTAAGCCATGCATGTCTAAGTACAAGCCTCAACATGGTGAAACC263720.21606550065142455No Hit
GCCCTATCAACTTTCGACGGTTAGTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTC235100.19261716670389015No Hit
GATCAGACGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAG217600.17827943630270734No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG183520.15035773047000392No Hit
GGTTAGTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGA172010.14092760036042595No Hit
CGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCA166450.13637229858725017No Hit
GCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGTGAAGCGG156630.12832678358498645No Hit
CTAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGGCCTATACCGTTCCGTCGCGGCAGAGTCTGTAGCGCGTGAC156190.1279662920777567No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCGGACATTTGGTTCATGTGCTCGGCCGATAGGCCGATGG152880.12525441278473298No Hit
TATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCT145550.11924895199383757No Hit
CGGTTAGTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAG140440.1150623347166922No Hit
CCGCGAATGGCTCATTAAATCAGTTATGGTTCCTTAGATCGTACAATCCTACTTGGATAACTGTAGTAATTCTAG131470.10771322376248592No Hit
ATCAGACGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGT131180.10747562708726631No Hit
GCCGCGACGAGTAGGAGGGCCGCTGCGGTGTGCGCGGAAGGCTTGGGCGTGAGCCCGGCTGGAGCCGCCGCAGGT122440.10031495487547559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG171050.060.02351847
CTCGTAT167700.059.27015343
GTATGCC174800.058.73558846
TCGTATG175350.058.5523244
TCTCGTA169150.058.37454242
ATGCCGT176700.057.96759448
CCGTCTT180350.056.88843551
CGTATGC181800.056.5511145
GCCGTCT181500.056.54745550
TGCCGTC181300.056.53395549
ATCTCGT177950.055.4483441
ACGATCT181050.054.05999438
TGCTTGA189500.053.90535459
GCTTGAA190250.053.71032760
CTTCTGC194900.052.53486655
CTGCTTG195150.052.238458
TCTGCTT196400.051.90507557
CCTATCG50550.050.4539381
TTCTGCT204400.049.95795456
GACGATC201350.048.6104937
Produced by FastQC (version 0.11.2)