FastQCFastQC Report
Mon 6 Mar 2017
HJNLLAFXX_n01_lbstara_m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNLLAFXX_n01_lbstara_m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12205066
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG176770.1448333011882115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG38250.021.10544447
TCTCGTA43350.019.41947642
ATGCCGT42350.019.22494548
CGTATGC44400.018.64856545
TGCCGTC44150.018.59727149
CTCGTAT46500.017.58444243
TCGTATG46600.017.54620644
GTATGCC47900.016.85351246
GCCGTCT48950.016.77363650
TATCTCG49400.016.20302240
ATCTCGT53550.015.52717941
CCGTCTT57050.014.573338551
GTATCTC57700.014.47020139
CGGATGT56950.014.23710134
CGTCTTC59800.014.19148952
ACGGATG60750.013.11964433
TCACGGA64200.012.62979531
CACGGAT63500.012.55157332
GCTTGAA67000.012.46052760
AGTCACG66200.012.14369129
Produced by FastQC (version 0.11.2)