FastQCFastQC Report
Mon 6 Mar 2017
HJNLLAFXX_n01_lbstara_fem.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNLLAFXX_n01_lbstara_fem.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555886
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG233684.203739615676596TruSeq Adapter, Index 3 (97% over 37bp)
CTTTCGACGGTTAGTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGA44050.7924286634309913No Hit
GACGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACG23090.41537293617756155No Hit
GATCAGACGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAG23010.4139337921804111No Hit
CGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGC22980.4133941131814796No Hit
TTTCGACGGTTAGTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGAG20340.3659023612755133No Hit
GTTAGTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAA18540.3335216213396272No Hit
GCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGTGAAGCGG16280.2928658034201257No Hit
CCTATCGAAAGGGAATCGGGTTAATATTCCCGAACCCGGACGCGGAGATTGTCCCCTCGGGGGCGAGTGCGGCAA14850.2671411044710606No Hit
ATCAGACGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGT14590.2624638864803215No Hit
GTACAATCCTACTTGGATAACTGTAGTAATTCTAGAGCTAATACATGCGAGCAAGCTCCGACCCTTACGGGAAAG12940.23278154153909256No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGACTAAGCCATGCATGTCTAAGTACAAGCCTCAACATGGTGAAACC11870.21353299057720468No Hit
GGTTAGTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGA11730.21101448858219132No Hit
AGACGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAAC10280.1849300036338386No Hit
GTTCCTTAGATCGTACAATCCTACTTGGATAACTGTAGTAATTCTAGAGCTAATACATGCGAGCAAGCTCCGACC9690.1743163166548537No Hit
CTTAGATCGTACAATCCTACTTGGATAACTGTAGTAATTCTAGAGCTAATACATGCGAGCAAGCTCCGACCCTTA9440.16981899166375838No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9390.16891952666553933No Hit
CGGTTAGTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAG9140.16442220167444405No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAATGGATGGCGCTGGAGC8790.15812594668691063No Hit
GCCCTATCAACTTTCGACGGTTAGTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTC8690.15632701669047253No Hit
CTAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGGCCTATACCGTTCCGTCGCGGCAGAGTCTGTAGCGCGTGAC8550.15380851469545914No Hit
GGTTCCTTAGATCGTACAATCCTACTTGGATAACTGTAGTAATTCTAGAGCTAATACATGCGAGCAAGCTCCGAC8370.15057044070187053No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCGGACATTTGGTTCATGTGCTCGGCCGATAGGCCGATGG8340.15003076170293908No Hit
GTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGG8200.14751225970792572No Hit
CTGCGGTGTGCGCGGAAGGCTTGGGCGTGAGCCCGGCTGGAGCCGCCGCAGGTGCAGATCTTGGTGGTAGTAGCA7990.1437345067154057No Hit
CGACGGTTAGTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCAT7700.13851760972573512No Hit
AGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGA7500.1349197497328589No Hit
CGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGGCTACCA7270.13078221074105123No Hit
GACAGGTTAGTTTTACCCTACTGATGACAGGCCGTTGCAATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGT7160.12880338774496927No Hit
TTCGACGGTTAGTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGAGC6960.12520552775209304No Hit
CAACTAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGGCCTATACCGTTCCGTCGCGGCAGAGTCTGTAGCGCGT6830.1228669187567235No Hit
GGAGTCTAACATGTGCGCGAGTCATTGGGCTCTACGAAACCTAAAGGCGTAATGAAAGTGAAGGGGTGCCTCTCG6810.12250713275743588No Hit
CTGAAAATGGATGGCGCTGGAGCGTCGGGCCTATACCGTTCCGTCGCGGCAGAGTCTGTAGCGCGTGACGCCGCG6410.11531141277168341No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAGCCTATTAACTAGTTCGCGGATTCCGTCTCGTCCG6350.11423205477382053No Hit
GGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCGGACATTTGGTTCATGTGCTCGGCCGATAGGCCGATGGT5920.10649665578913663No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG26950.066.182347
CTCGTAT27150.065.839643
TCGTATG27300.065.7247444
TCTCGTA27100.065.70640642
GTATGCC27200.065.70083646
TATCTCG27350.065.48431440
TGCCGTC27350.065.340549
CCGTCTT27200.065.32033551
ATGCCGT27350.065.2143648
TCTGCTT27100.065.1735957
ATCTCGT27450.064.86861441
GCCGTCT27550.064.7409450
TGCTTGA27750.064.6414959
GCTTGAA27700.064.6336360
CTATCTC27700.064.5323239
GCTATCT27550.064.25738538
CTGCTTG27600.064.24287458
CGCTATC27500.064.1117137
TTCGCTA27750.063.90712435
CTTCTGC27750.063.7713155
Produced by FastQC (version 0.11.2)