FastQCFastQC Report
Mon 6 Mar 2017
HJNLLAFXX_n01_lbhearte_fem.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNLLAFXX_n01_lbhearte_fem.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5111223
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1664643.2568330515025465TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG127400.24925541303911022No Hit
CGGCGAGTGAAGCGGGAAGAGCCCAGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGGAATCTCG84200.16473552415928633TruSeq Adapter, Index 7 (97% over 36bp)
GTAACGGCGAGTGAAGCGGGAAGAGCCCAGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGGAAT78820.15420966762749347TruSeq Adapter, Index 7 (97% over 36bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG67000.1310840869200972TruSeq Adapter, Index 1 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGGAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG59670.11674309651525672TruSeq Adapter, Index 7 (97% over 36bp)
CTAGTAACGGCGAGTGAAGCGGGAAGAGCCCAGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGG59260.1159409401624621TruSeq Adapter, Index 7 (97% over 36bp)
CGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGC52530.10277383710317473No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG227700.060.45269447
CGTATGC230400.059.81971445
ATGCCGT230150.059.7941748
GCCGTCT229900.059.69354250
TGCCGTC230500.059.58363749
GTATGCC233000.059.15162346
CGTCTTC232050.059.1244452
CCGTCTT232900.059.0431251
TCGTATG234400.058.8436344
CTGCTTG231000.058.42246658
GCTTGAA234550.058.08299660
TGCTTGA233500.057.9600559
CTCGTAT227650.057.5900243
GTCTTCT237200.057.4620553
CTTCTGC238500.057.10601455
TCTGCTT240200.056.37151357
TTCTGCT242050.055.9834256
CTTGAAA245900.055.6691661
TCTCGTA235950.055.54901542
TCTTCTG245600.055.48269754
Produced by FastQC (version 0.11.2)