FastQCFastQC Report
Mon 6 Mar 2017
HJNLLAFXX_n01_bay54c_fem.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNLLAFXX_n01_bay54c_fem.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10652264
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1315261.234723435318539TruSeq Adapter, Index 8 (97% over 36bp)
GACGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACG275490.258621078110719No Hit
GCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGTGAAGCGG252930.23744248171093016No Hit
CGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGC252760.23728289122387503No Hit
GATCAGACGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAG251040.2356682110019053No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG195840.18384824108752842TruSeq Adapter, Index 16 (97% over 35bp)
CCTATCGAAAGGGAATCGGGTTAATATTCCCGAACCCGGACGCGGAGATTGTCCCCTCGGGGGCGAGTGCGGCAA166770.15655826780109844No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG152230.14290858732002887No Hit
ATCAGACGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGT122310.11482066159832313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG220200.050.74643747
ATGCCGT223200.049.9409448
TCTCGTA214650.049.92348542
TGCCGTC223200.049.7549949
CTCGTAT216900.049.5805843
CGTATGC226500.049.51819245
GTATGCC228250.049.33456846
GCCGTCT221600.049.32002650
TCGTATG224850.049.25224744
CCGTCTT222600.049.06751
CGGATCT219600.048.89196838
TCGGATC222600.048.6362537
ATCTCGT222750.047.99944341
CGTCTTC230850.047.19325352
GCTTGAA234600.046.952960
GGATCTC229950.046.6622239
CTCGGAT241600.046.252636
TGCTTGA237200.045.81307259
CTTCTGC239100.045.23217455
CTGCTTG239000.045.10696858
Produced by FastQC (version 0.11.2)