FastQCFastQC Report
Mon 6 Mar 2017
HJNLLAFXX_n01_bay46d-fem.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNLLAFXX_n01_bay46d-fem.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8526273
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG3433534.0269998392028965TruSeq Adapter, Index 16 (97% over 38bp)
CGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGC1531621.7963534594775465No Hit
GACGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACG1293701.5173100837845561No Hit
GATCAGACGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAG1133791.3297603771307815No Hit
ATCAGACGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGT775680.9097527137589894No Hit
GCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGTGAAGCGG682310.8002441394968236No Hit
CTAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGGCCTATACCGTTCCGTCGCGGCAGAGTCTGTAGCGCGTGAC614270.720443739017036No Hit
CCTATCGAAAGGGAATCGGGTTAATATTCCCGAACCCGGACGCGGAGATTGTCCCCTCGGGGGCGAGTGCGGCAA596240.6992973365971275No Hit
CCTCCTTCGTGCTAGGGATTGGGGCTTGTAATTCTTCCCCATGAACGAGGAATTCCCAGTAAGCGCGAGTCATCA488670.5731343577668696No Hit
GCCGCGACGAGTAGGAGGGCCGCTGCGGTGTGCGCGGAAGGCTTGGGCGTGAGCCCGGCTGGAGCCGCCGCAGGT356640.41828358064537696No Hit
GGCCGCTGCGGTGTGCGCGGAAGGCTTGGGCGTGAGCCCGGCTGGAGCCGCCGCAGGTGCAGATCTTGGTGGTAG328490.3852679828572226No Hit
TCAGACGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTA304080.35663882683559395No Hit
AGACGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAAC285420.3347535318186504No Hit
CTGAAAATGGATGGCGCTGGAGCGTCGGGCCTATACCGTTCCGTCGCGGCAGAGTCTGTAGCGCGTGACGCCGCG284610.3338035270510339No Hit
CTTTCGACGGTTAGTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGA277010.32488990207092827No Hit
CAACTAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGGCCTATACCGTTCCGTCGCGGCAGAGTCTGTAGCGCGT275220.322790508819035No Hit
CAGACGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAA250790.2941378958895639No Hit
GAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCG230190.26997728081190925No Hit
CTGCGGTGTGCGCGGAAGGCTTGGGCGTGAGCCCGGCTGGAGCCGCCGCAGGTGCAGATCTTGGTGGTAGTAGCA221380.2596445129073395No Hit
CGAGTAGGAGGGCCGCTGCGGTGTGCGCGGAAGGCTTGGGCGTGAGCCCGGCTGGAGCCGCCGCAGGTGCAGATC218340.2560790629152972No Hit
GGCCGATGGTGCGAGGCTACCATCCGAGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAAAAGTGTA205130.24058577528540312No Hit
TGAGATCAGACGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCC196060.22994806757888234No Hit
CGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGTGAAGCG195090.22881040754852677No Hit
AGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGA194450.22805978649757053No Hit
CGGGGGCATTCGTATTGCGGTGTTAGAGGTGAAATTCTTGGATCGCCGCAAGACGACCGGCTGCGAAAGCGTTTG183690.21543997007836838No Hit
GTTAGTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAA183320.21500601728328428No Hit
CGGCAGAGTCTGTAGCGCGTGACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGTGCGCGGAAGGCTTGGGCGTGA183240.21491218965191475No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAATGGATGGCGCTGGAGC176820.20738252223450973No Hit
GCCGCTGCGGTGTGCGCGGAAGGCTTGGGCGTGAGCCCGGCTGGAGCCGCCGCAGGTGCAGATCTTGGTGGTAGT174940.20517757289732572No Hit
CGCCGCGACGAGTAGGAGGGCCGCTGCGGTGTGCGCGGAAGGCTTGGGCGTGAGCCCGGCTGGAGCCGCCGCAGG173430.20340657635522577No Hit
TGAAAATGGATGGCGCTGGAGCGTCGGGCCTATACCGTTCCGTCGCGGCAGAGTCTGTAGCGCGTGACGCCGCGA171130.2007090319533517No Hit
GCCGAATCAACTAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGGCCTATACCGTTCCGTCGCGGCAGAGTCTGT165560.194176283119248No Hit
CCGTTCCGTCGCGGCAGAGTCTGTAGCGCGTGACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGTGCGCGGAAGG165380.19396517094866655No Hit
CTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGTGAAGCGGG164890.19339047670652817No Hit
GACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAG162720.19084540220562957No Hit
CGTTCCGTCGCGGCAGAGTCTGTAGCGCGTGACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGTGCGCGGAAGGC157830.1851101882381669No Hit
ACGAGACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGG157310.18450030863426495No Hit
CGAAGCCAGAGGAAACTCTGGTGGAGGACCGTAGCGATTCTGACGTGCAAATCGATCGTCGAACTTGGGTATAGG153750.18032497903832073No Hit
TCGAAAGGGAATCGGGTTAATATTCCCGAACCCGGACGCGGAGATTGTCCCCTCGGGGGCGAGTGCGGCAACGCA152650.17903484910698966No Hit
GGAAACTCTGGTGGAGGACCGTAGCGATTCTGACGTGCAAATCGATCGTCGAACTTGGGTATAGGGGCGAAAGAC149650.1755163129306322No Hit
TTTCGACGGTTAGTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGAG148720.17442556671596138No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG144350.16930023235240063No Hit
CTCTGGTGGAGGACCGTAGCGATTCTGACGTGCAAATCGATCGTCGAACTTGGGTATAGGGGCGAAAGACTAATC143810.16866689584065628No Hit
GGATGGCGCTGGAGCGTCGGGCCTATACCGTTCCGTCGCGGCAGAGTCTGTAGCGCGTGACGCCGCGACGAGTAG141390.1658286099917279No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCGGACATTTGGTTCATGTGCTCGGCCGATAGGCCGATGG139570.16369403137807106No Hit
GTCGAAGCCAGAGGAAACTCTGGTGGAGGACCGTAGCGATTCTGACGTGCAAATCGATCGTCGAACTTGGGTATA138390.16231007381537046No Hit
GGCAGAGTCTGTAGCGCGTGACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGTGCGCGGAAGGCTTGGGCGTGAG128900.1511797710441596No Hit
CCTGAAAATGGATGGCGCTGGAGCGTCGGGCCTATACCGTTCCGTCGCGGCAGAGTCTGTAGCGCGTGACGCCGC125670.14739148042761474No Hit
CGCTGGAGCGTCGGGCCTATACCGTTCCGTCGCGGCAGAGTCTGTAGCGCGTGACGCCGCGACGAGTAGGAGGGC122820.14404887106007513No Hit
CGCGACGAGTAGGAGGGCCGCTGCGGTGTGCGCGGAAGGCTTGGGCGTGAGCCCGGCTGGAGCCGCCGCAGGTGC122520.14369701744243937No Hit
ATCAACTAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGGCCTATACCGTTCCGTCGCGGCAGAGTCTGTAGCGC120870.14176182254544278No Hit
GAACTATGCCTGAGTAGGTCGAAGCCAGAGGAAACTCTGGTGGAGGACCGTAGCGATTCTGACGTGCAAATCGAT115960.1360031516701377No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTCAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG114310.1340679567731411TruSeq Adapter, Index 16 (97% over 38bp)
AATCAACTAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGGCCTATACCGTTCCGTCGCGGCAGAGTCTGTAGCG114200.13393894378000798No Hit
ACTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGT111580.13086608885265577No Hit
ATCGAAAGGGAATCGGGTTAATATTCCCGAACCCGGACGCGGAGATTGTCCCCTCGGGGGCGAGTGCGGCAACGC111260.13049077832717765No Hit
CCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGTGAAGC108130.1268197722498447No Hit
GTACAATCCTACTTGGATAACTGTAGTAATTCTAGAGCTAATACATGCGAGCAAGCTCCGACCCTTACGGGAAAG105520.12375864577641368No Hit
CCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGTGAAG104940.12307839544898458No Hit
CGCTGCGGTGTGCGCGGAAGGCTTGGGCGTGAGCCCGGCTGGAGCCGCCGCAGGTGCAGATCTTGGTGGTAGTAG104080.1220697484117621No Hit
TGGCGCTGGAGCGTCGGGCCTATACCGTTCCGTCGCGGCAGAGTCTGTAGCGCGTGACGCCGCGACGAGTAGGAG103610.12151851107746608No Hit
CGAAAGGGAATCGGGTTAATATTCCCGAACCCGGACGCGGAGATTGTCCCCTCGGGGGCGAGTGCGGCAACGCAA102010.11964195845007543No Hit
CCGCGACGAGTAGGAGGGCCGCTGCGGTGTGCGCGGAAGGCTTGGGCGTGAGCCCGGCTGGAGCCGCCGCAGGTG101830.11943084627949398No Hit
CTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGTG100060.11735490993544308No Hit
GGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGGCCTATACCG99200.1163462628982206No Hit
CTCCTTCGTGCTAGGGATTGGGGCTTGTAATTCTTCCCCATGAACGAGGAATTCCCAGTAAGCGCGAGTCATCAG98640.11568946947863386No Hit
ATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGTGAAGCGGGAAGA97340.11416477046887895No Hit
TGCCGAATCAACTAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGGCCTATACCGTTCCGTCGCGGCAGAGTCTG97330.11415304201495777No Hit
CCGATCTCGGCGCGCCCGGTGGTGACGAGCCACAGCAACTGACGGCATTCAGGGGTCCGCCGAGCAGGGCGGGCT97300.11411785665319418No Hit
GCCAGAGGAAACTCTGGTGGAGGACCGTAGCGATTCTGACGTGCAAATCGATCGTCGAACTTGGGTATAGGGGCG96590.1132851364247896No Hit
CTGTAGCGCGTGACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGTGCGCGGAAGGCTTGGGCGTGAGCCCGGCTG96590.1132851364247896No Hit
TGTAGCGCGTGACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGTGCGCGGAAGGCTTGGGCGTGAGCCCGGCTGG96010.11260488609736048No Hit
GGGGCTTGTAATTCTTCCCCATGAACGAGGAATTCCCAGTAAGCGCGAGTCATCAGCTCGCGTTGATTACGTCCC96010.11260488609736048No Hit
TACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGTGA95580.11210056257874923No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGGCCTA93420.10956721653177184No Hit
GTTATACGCTCACCGTGGTCGTAACGGGTAGCGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCATGAGAAACGG93320.10944993199255994No Hit
GGTACCGTTTGCGCCTCGGCGTTGGGAGGCAACGATATGCGGGCGTCGACCGATCTCGGCGCGCCCGGTGGTGAC91620.10745609482595737No Hit
GCTGGAGCGTCGGGCCTATACCGTTCCGTCGCGGCAGAGTCTGTAGCGCGTGACGCCGCGACGAGTAGGAGGGCC91520.10733881028674545No Hit
GGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGTGAAGCGGGAAGAGCCCAGCACAGATCGGAAGAGCA90300.10590793890836007No Hit
CGCGGCAGAGTCTGTAGCGCGTGACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGTGCGCGGAAGGCTTGGGCGT88980.10435978299076279No Hit
CGCGGAGTCCGGTGCACTGGGGTCGGCCCTTGAAAATCCTCGGGAGAGTGTGTGATTTTCGCGCCGGACCGTACC87690.10284681243492907No Hit
GTCTGTAGCGCGTGACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGTGCGCGGAAGGCTTGGGCGTGAGCCCGGC86980.10201409220652446No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCTT412900.064.9940157
TGCTTGA414150.064.8993359
GCTTGAA416650.064.6834260
CTGCTTG417100.064.2989658
TTCTGCT419600.064.252256
CTTCTGC422200.063.96311655
CCGTCTT424650.063.57269351
TATGCCG427250.063.23464647
ATGCCGT427450.063.1404948
TGCCGTC427550.063.0369649
CCTATCG78950.062.992631
GCCGTCT429500.062.78326450
GTATGCC430850.062.77834746
TCGTATG434450.062.3216844
CGTATGC436550.061.98237245
CTCGTAT419450.061.70485743
AAAAAAG433400.060.94203669
TCTCGTA424800.060.78154842
GTCTTCT445300.060.6844753
CGTCTTC449850.060.14004552
Produced by FastQC (version 0.11.2)