FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2309570
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTACCAAGGCCGTCTACAACAAGGCACG74140.3210121364583018No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTTATTCCCCGTTACCCGTTGAAACCA50020.21657711175673394No Hit
GCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAATTCCAGCTCCGCTTCATTGAATAAGTAAAGAAAC46000.1991712743064726No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAGTGCACCGTTGCTAGCCTGCTATGGT42140.18245820650597297No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTACACCCTCTATGTCTCTTCACAATGTC40760.1764830682767788No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCC35570.15401135276263547No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34440.14911866711119387No Hit
ATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAGTGCACCGTTGCT32860.1422775668197976No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTAT32360.14011266166429248No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTGTTGAGC30420.13171282966093256No Hit
GTATTTATTAGATAAAAAATCAATGTCTTCGGACTCTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTT30150.13054378087695978No Hit
AATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCTACTATCTAGCGAAACCACAGCCAAG29200.12643046108150002No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGC28740.1244387483384353No Hit
TCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGT26710.11564923340708444No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTAATGAGCCATTCGCAGTTTCACTGTA26020.11266166429248735No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATT25420.11006377810588118No Hit
GTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTG23850.10326597591759504No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCTTTGGAAGAGATGTATTTATTAGATA23720.10270310057716371No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCG24900.028.816599
GCAAGCG24450.028.64274225
GGTCTAC24700.028.35836234
TAAGTTG26000.027.996685
CAAGCGA25250.027.87187626
TGTAGCA25200.027.7902821
TTCTAAG25850.027.4924552
TGATCGT26100.027.49228510
AAGCGAC25700.027.25020427
TTGTAGC25800.027.14399520
GTTAATT26300.027.02088215
GCGACGG25950.026.9894329
GTTCTAA26500.026.9494631
GATCGTT26600.026.84582311
TAGCAAG26800.026.38860923
ATCTAAG8250.026.344145
CTATGCG3700.026.1068063
ATTGTAG27250.025.95283319
GTCATAT9400.025.6918751
TCTCGGG951.050185E-525.42473835
Produced by FastQC (version 0.11.2)