FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2211242
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAGTGCACCGTTGCTAGCCTGCTATGGT34750.15715150128298938No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28510.1289320662324612No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGCCATAATCCAGCG25440.1150484659752302No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCACG7000.029.0758177
CCACGCA7750.026.707159
CCCACGC8500.023.5389488
GGGGCTC5200.021.89116710
TAAGGGG5500.020.6985075
CACGCAA10350.020.33048810
ACGCAAA10550.019.94507611
GTCCTAA5400.019.8072641
ATTCGCC19250.019.35357316
CATTGCA21550.018.7290849
ATGACAT21850.018.314475
AATTCGC20700.018.16412515
CAATCGG8750.018.13870836
CGACGGG8150.017.77987149
CATATGC6150.017.3893833
GACGGGC8550.016.94806350
GCAAGCA22450.016.90339524
CGGGCGG8600.016.84952752
GGGCTCA6350.016.84016411
GCTCATG6200.016.69083613
Produced by FastQC (version 0.11.2)