FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2138786
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24750.11571985229003744No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAGTGCACCGTTGCTAGCCTGCTATGGT22400.10473231075946822No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCACG4300.030.4867617
CGCGTGT1501.8189894E-1229.89981869
ACGCGTG2900.027.36190269
GTCATAT6400.024.7984641
CCACGCA5750.024.5986989
GACGCGC2600.021.23113438
CCCACGC6500.020.6989068
GCATGTC5550.020.51722531
GGGCTCA4550.020.47144711
CGGCCCT850.00411756520.29731634
CGCGCAT2553.6379788E-1220.29351840
CATATGC6150.019.6335663
CGAGTGT1601.3636309E-619.4052248
CCCCGTG1801.9800427E-719.16968730
TCATATG6850.019.1385692
GTCCTAA5050.019.1299381
GCTCATG4800.018.68607513
TGACGCG2801.2732926E-1118.48289537
GGGGCTC4900.018.30515510
TAAGGGG5100.018.2641665
Produced by FastQC (version 0.11.2)