FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169756
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGTA150.002353156268.9920566
GTAGGCC200.007351642551.7440343
CCGCTTT200.007351642551.74403461
GACCCTA200.007351642551.7440346
GCCGCTT200.007351642551.74403460
TCAGCCG306.299765E-445.99469857
ATAGGGA450.00461431630.6631328
CAGCCGC450.00461431630.66313258
GTATAGA450.00461431630.6631321
AGCTATC700.001330064724.6400177
TCTTAGA851.4373627E-424.3501342
GCTGTGT750.001987404222.9973498
GGTTAAG750.001987404222.9973491
ATATGCT953.0531315E-421.7869634
TTTACAC850.00410870520.2917793
GCAGACT850.00410870520.29177913
GCTCAAA850.00410870520.29177911
ATCTAAT1108.2036556E-418.8160131
ACAGAGC1852.6133603E-718.64658
ACACAGA1653.8143626E-516.7253446
Produced by FastQC (version 0.11.2)