FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences610374
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6480.1061644172261597No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGGC850.004117974520.29414250
CTGTGCA950.00783029718.1579179
CCGACGA1150.001109403418.00002369
TAGCTAG1150.001109403418.0000235
TTGAGCG1200.00147569217.2500214
TACACTG1402.0805439E-417.250025
GCTGGTC1653.8367936E-516.7272938
GCCGACG1250.001939191316.5600268
TATACAG4750.015.9789675
TAATACT5200.015.9230954
GTATTAG3101.0768417E-915.5819411
CTGGTCA1350.003240691315.3333519
TATAGGG1605.7525234E-415.0937682
GTGTAAG1657.260046E-414.6375811
ATATAGG2901.2546298E-714.2770481
TCCTAGA1450.005211137214.2758792
TAGGTAG1709.1068627E-414.2058995
CTTACAC1709.1068627E-414.2058993
ATACAGG2454.946698E-614.0816496
CCAATCC2001.9790421E-413.8000169
Produced by FastQC (version 0.11.2)