FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3838008
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46470.12107843443786463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG8100.013.6283975
GTATTAG16050.013.3272871
ACCGCGC2851.6145605E-613.3147598
GTATATA40800.013.1067981
GTATAGG13100.012.9047581
CCTACAC6500.012.7373093
CTAATAC18800.012.4775943
TCCGCAC6650.012.45146264
TATACGG13500.012.2655575
TCTATAC17500.012.0246283
TATACTG22800.011.9528675
GTATAGA17700.011.5001591
TACACCG6700.011.3273335
CCGCACC8000.011.21265565
TAGGGGT4351.247081E-711.1024454
ACGGTGT11500.011.099148
ACTGTGC8150.011.0052838
TATACAG23700.010.7711785
ACCCGCG2903.7044243E-410.70885833
TAATACT30750.010.5453874
Produced by FastQC (version 0.11.2)