FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658811
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19170.11556470266956272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGA1709.116575E-414.20629229
TACCGTG3355.0298695E-813.3876387
GTATATA19450.013.3041271
GACTGAC2102.9972545E-413.1424457
GGCTGTG2651.1154856E-513.0184597
GTACTAG4601.2732926E-1012.7507521
GTATAAT12450.012.747741
TATACTG10900.012.3436485
ATACGAC3408.7206354E-712.1760873
TATATAC22400.012.1670273
CTAGTAC4853.110472E-1012.0924043
GTATTAG7500.011.9607051
TATACAG12300.011.7801175
CATATAC12650.011.7269043
TAGGACT2651.6076802E-411.7166134
TCTATAC7450.011.5768163
GTATAGT8850.011.3057521
ATTAGGC4301.0603253E-711.2322053
GCTATAG3401.1573333E-511.1614133
TATACCG4652.6131602E-811.1286825
Produced by FastQC (version 0.11.2)