FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1871504
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19610.10478203626601922No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC1452.7259052E-416.6532968
CTAGGAC2955.366019E-1016.3710373
GTCCTAG2705.246693E-815.3348841
CTAATAC7950.014.7530563
CCGCACC3051.4666512E-814.7044465
GTATATG10600.014.6477061
GTATAGA9300.014.4692041
GTACTAG4505.456968E-1213.8013941
CGCACCT3001.9077743E-713.79955166
GTATAGG5050.013.6647471
CCCCTAA3557.3159754E-913.6070091
TACACAG5650.013.4321155
TCGGGGC2153.6544527E-412.8378258
GTATAAG9150.012.8209691
GTATATA17800.012.7934271
TGCGGGC1900.002109512.71045247
TACACCG3551.0747499E-712.6323795
TCCGCAC3601.2883902E-712.45792864
ACTATGC6650.012.4497258
GTATAAC8100.012.3531011
Produced by FastQC (version 0.11.2)