FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77
Sequences flagged as poor quality0
Sequence length75
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTTATTACGCATGGCTTTTATTCATTGCTTTCTAACTGTGATGGGAGTTTTTTTCTTGTTGCATAAATGAACG11.2987012987012987No Hit
GTTATCTATGGGCGGAAGTCTTTGCAACTGATATGTATCACACCAAATTCGCTAAGGATCCGTTAAATGCCAAGA11.2987012987012987No Hit
GTTAAGAAGACGACAACACGAACAGTGGCGTGAATACGAAAAACCAATAATAGTTACTGACAGTATGTCACCCCA11.2987012987012987No Hit
GGGTAAGACAAACTTGGACCAATTTGCCACAGGATTAGTCGGCACACGGTCTCCATATGGGAAAACACCTTGCGC11.2987012987012987No Hit
GCGTGGAGGTAAGTATTGCCGCTAATACTTGAGCCTCTGATGTAAATGTTTGAGGGCGGAGCTTGATATACAGAT11.2987012987012987No Hit
GATCATTATGAGTGAGACTGGAAGATGCAGAGGCAAGACGATGTCCAATGTTTCCAAATGCACTCTTACGCTTAT11.2987012987012987No Hit
TGCGAGTTATGTGGCAAGCGACTTTTATGCATGTGGTTTTCTCTGTCTCTTATACACATCTGACGCTGCCGACGA11.2987012987012987No Hit
GTATTGACTCCTCACTACGCGGAAAGAATTCTGCTGTGATTAAGAGAAATTATTGGTTAAGATGAGGAATTTTCT11.2987012987012987No Hit
GTTCCCTCATCCGAGCTTATCGGAATCGTTTAAGCAAGCCGTCCAGTTGGCAATGGCAAATGGAACATCACCTGG11.2987012987012987No Hit
GGCAATTCGAAGCAACTCAACTGGGTTTTGACCAAACAATGAAGAGGTAAGAGATTCTTAATAAGAAATGTCACG11.2987012987012987No Hit
AATGAGTTGGAAACTATCGCAGCCAGTGTCGAAGAAATGAGCGCCGCTGGTACTGCATCTTGGGGCGAATTATTC11.2987012987012987No Hit
TCCCAGATGAACAACATACAGCCAACTTTGTCCCCAGATCACAATGCGAAGGGCCAGTCCCCTCACACTCTTTTC11.2987012987012987No Hit
CCTTATTCGTATCCATCTATATTGTGAGGAGCAAAGAAATCACCTCGTTCTAGCATGGATTCTGACTTAGAGGAG11.2987012987012987No Hit
CCTTTTTATTGTGTGATAGCTTGGTACCGCTTGATGCTTCTCGGGCATAATAAAGAGTGGCTCTTATAGAGATCT11.2987012987012987No Hit
CCAGGGATGAGTCTGGTGCCGTGGGCCGCTGGTATACCAGGCATCCGCTGGCTCGTCGCCACATCCGACGCTGCC11.2987012987012987No Hit
TCACGTAATTGATCAATAGGTAAAACACCATTACACACGAGCTCACCCTTGTTATATGCAAAGTTTGGGAAGACA11.2987012987012987No Hit
TTGCAGATCGAACCGCTCGGAATATGATTGTGAAGGGAGTAATCGAATCTAACGAAAGATAAAAAGAGCCCTTGC11.2987012987012987No Hit
CACAAGAAGTGTGCAATTCAGTCCGAATTGAGTACAGAGGGACTATGTTTCAAAGGTCTGGAGCTGCTCATCACA11.2987012987012987No Hit
GTCTATTTACTTCATTATAAGCATTAATTGCGGCTTTTAGCATTTCAATAGGTGCCACTGATCCGGTATTTAACC11.2987012987012987No Hit
ACCTAATACTATGCAAAAATGTAAAATCATCACACAAAACATAAACAATCAAAATCAGCCATTTCCGCACCTTTT11.2987012987012987No Hit
CGTAATACCCGTTTGATGGCCTACACCTAGTAAATCTTCAAATGGTACGAATCCCATATTTTCTACTTTGTTTCC11.2987012987012987No Hit
AAATAGAAATTGGAAAATTAATTACATCTCCGCATAATTGAATAACTAAATAAACAAAAAATCTACTTCTAAAAA11.2987012987012987No Hit
GAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAG11.2987012987012987No Hit
GCCACCTGGGGCACCACTTCTCTTCTTCTTGTTTTTTGGTTTTGGTGGGCCTACCACCTTAGGCTTAGGTAAAAA11.2987012987012987No Hit
GTATTGGAATGAGCGGATTAGTGATGCTATTGTGATTGGATTGTTAGAATGTATCACGGGCGAGGGACATGGTGG11.2987012987012987No Hit
TCTCCTTTCTATAAAGGGCGTCTTTCACTTCACTTGTGCCATGTTACAAAGCTCCAAACGCACTTCTAACTGAGT11.2987012987012987No Hit
GATGACTATCAATTAAATGACAGAAACGTGACCAAAGGGGACATAGTCAGAATGTTGGAAACCGCTGGTTTTTCA11.2987012987012987No Hit
AATATTCATCTAGGATCTTATTAATATTAGAGTGATTAAGATTCTGTAAAAATATCAAAAACGACATCTTTACTC11.2987012987012987No Hit
TGCCAAAACAAAGACAGCCATAGCTTTTTGTTCATCGGTGGTATCATTAGAATGATAAAATGGAAGATTATTGTG11.2987012987012987No Hit
ATGTAAGACATATTCCCGTTAAGTCATTAGTTTTTCATTCTAAACATCTGTCTCTTATACACATCTGACGCTGCC11.2987012987012987No Hit
GCCTTAATCTGTCTCCACAATTCGTTGATGGATGACCCGGTAGTGGAATAAGAGAAATGAGGATATTGAGAAAAG11.2987012987012987No Hit
CATTATTATTGTGAAGTTCTCCCGCGACTACATCGAGCGATGGATTTCTCGAAGATAAGTGAGCGGCAGTCTCTT11.2987012987012987No Hit
TTATCAAGGAGCTGTCCTAACTTCAATGTCGGTATTAAAAAGACCTTGACCAACATTGAAACCGCAAGGCACGAT11.2987012987012987No Hit
TATGAGTTGGAAACTATCGAATCAAGTGTCGAATAAATGAGATCCGCTTGTACTGCATCTTGGTGCGAATTATTC11.2987012987012987No Hit
GAGAATATGAAGGTGAATTAGATAATTAAAGGGAAGGAACTCGGCAAAGATAGCTCATAAGTTAGTCAATAAAGA11.2987012987012987No Hit
GGTTGGGAGATAATATTTATGGTATGGGGTGCTAGTGCGGATGGGGCGCTCTTATTGTTGATTTCTTCGCTCGTC11.2987012987012987No Hit
AAAGAGGATAGAAGATCGAGCAAAAAAATTTTTTTAATATGGGGTCAGTGGCGATATTATACTATAGGAGTTAAA11.2987012987012987No Hit
GAGTAGAATCGTCGATTAAACTTCCTTCTCCTTTTAAAAATTAAAAACAGCAAATAGTTAGATGAATATATTAAA11.2987012987012987No Hit
ACAGATTTAGATCAGTACAAATTTTCCAAAAGGATAATGTCCAAGTAATTGGTCAAGGTTTTAGTGACAGACATG11.2987012987012987No Hit
GTAATAGGGGGAGGGGGTGGGTGATAAGAACCAAACTATTCAATAAATATAGAGCACACATTAGTTAATATTTAA11.2987012987012987No Hit
GACCATAGTATCCTTGATACATGGACTCATAATCAGTTAAATAGCCATTCAATCCCTTTGAGCTATCAACTTCAT11.2987012987012987No Hit
GCTTACATCTATGGTGATTCTATACTTCCCCGGTTACTTATAGTTTTTTGTCCTAAAAATATTTTCTATCTAATC11.2987012987012987No Hit
TACCGAGCCTAACAATGGTAACAATGAAACTGCGGAGCCTCAGGGACCAAATCAGAGAATTGATTTGGGTGCCTT11.2987012987012987No Hit
CTTAAAGGCCCTTTTTGATCATTTTCTTTTACCTTTATTTGCCACCCGCACCTTTTGTCACACTACACGAGAATA11.2987012987012987No Hit
ATCCTGATGATAGAGGCGGATGGGGAATCAGTCCGAGAGGTGCAGGCTTCACGTTTGGTCAAGACATCAGTGAGC11.2987012987012987No Hit
TAGACGTTCTGGGCCGCACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAAT11.2987012987012987No Hit
CAGATAATAATATTCACGAGATAACATTTGTTCACCCATACCAGCACCAATCTTAGTAGTGAAATTTAGAATTGT11.2987012987012987No Hit
CCCTTGACGAAAGATAACCACTCTGGAATCAAGTCTTCAGCTTCATCAGTGATGAAGACACGACGAACGTACAAC11.2987012987012987No Hit
CATTAATATCGACAGTTTGTATTCCTTTGCCTTTTGCCTTAGCAATAGCAAAAAGCTCCCGAGCGCTTTCTATAT11.2987012987012987No Hit
AAATATCTTAGTCCCTCGTCTAAAATGGCTTAACAAACCATTAAAAAAAATTCACTTTTATTACTTCTATGCAAA11.2987012987012987No Hit
TTTGAAGATGATGACGTTTACAACTATAACGACAACGACGACGATGACGAAAGAATCGAATTCGAAGATGATGAC11.2987012987012987No Hit
AAGCCAAGGAATGGTTGTTGAGATTTCCAACGGCAATACTTCTTCAAAGACTGTTGTCACTCTTTCAGAACCTGT11.2987012987012987No Hit
GGTTTGGATATTAAATTTGTTAAATAAAATAATACAATAAATTCAAACTAAATACTGTTTCTTTTTTCACCTAAC11.2987012987012987No Hit
TTCTTATTCCTTCCCGCTTTCCTGCACTAACATTTTGCCGCATTACACTATATGATCGTAGTACATCTTACAACA11.2987012987012987No Hit
GGTTTAAAAAAACTAAATAATCCGCGTGATAACGTTCTCACCAGATTATGAAATCAAATAACAATGCCCCACCTG11.2987012987012987No Hit
CTGTTGTATCTGCCGCTAATTCGAATGTTTACGTAGGTTCCCGTGTTCTTTACTCTTTAGCTAGGACTGGTAACG11.2987012987012987No Hit
ATTTGATTCCACCATTCATCTTGGCGTTGTAGTTGAAGCGCTGACAATCGGTGTCGTCTGTCGTGACCCTGTCTC11.2987012987012987No Hit
TGCCTGAATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGGTTCTGACGTGCAAATCGATCGTCGA11.2987012987012987No Hit
GATTATGGTAAACGTTTCAATTGGTGTGTACCTTTTAAGTCTTGGTGTTTTCCCATTGTGGTGGTCTTCTCTATC11.2987012987012987No Hit
TTCTTGATTAGTGGAACCAACGGCAAATTTTGGCCGTCCTCACCACCATCAACTAAAAACAAATCATCGGCATCA11.2987012987012987No Hit
ATATATTTTTCTTGGGTGGTGAAAAAGACAAGTCTTTCAAACTATGCGACCTAGAAAAAAAATATTTTACCGCAA11.2987012987012987No Hit
TCGCAAAGGGTGTGACATGGCTACACTTCTAGATTGGTTCTGTGGCACACTTAGATGCTTATCATCACCGTCATC11.2987012987012987No Hit
TGCTTCTTTTACCGATATTGCTTCCTGTGAACGCTACCAACGGTAACAACCTTCAAGGCTTTGAACTGCTATCAT11.2987012987012987No Hit
TCAGGAGTGTTGGTGATCCATGGGCTTGGGTATGAGGGGTGCCTCTGCAGTTGGGGGGTGGGGGATGGATTCGTG11.2987012987012987No Hit
CTTAAAAATTTTTGAAAATGCGATGCCTAATTAATCAATAATATTATTATATTTATAAATAGAAGTACACTACGC11.2987012987012987No Hit
CTACCTTTATTTTATGTTTACTTTTTATAGATTGTCTTTTTATCCTACTCTTTCCCACTTGTCTCTCGCTACTGC11.2987012987012987No Hit
GAATTACAAGTGGATGCGCCAGAATTTGACACGCTTGATTCGTCTTCAATAGACAGAATTTGAGCCTTTCTTGCT11.2987012987012987No Hit
ACTATTAGTGCTCCTAACAGCTTTACTTCCGACATACCGCAATACAACCACGGTTCCCTCGGCAACAGCGTCTCC11.2987012987012987No Hit
GGATAGGAAAAGGTCTTTTGAAAAAATTGTCATTTCCGTCATGGTCGGGAAAAATGTACAAAAGTTCCTGACATT11.2987012987012987No Hit
GATTATAGTTGATCCTCATTAGGACATCTATCGTAACAATGGGAATGTTCATATCATACATTAATTTATACGAGT11.2987012987012987No Hit
GTACTTTCAGTGGTAGTAGCATTAGTGATGGAGTTGGTAGTGTCAGTGGTAGTAGCAATTGTCCTGGCTTTTGTA11.2987012987012987No Hit
GTGGGTGAATGCACAGTAAGGTGGAAAGAGGCATCATTCAAGAATAGTCGCAAGCGGAAAAAGGTGAGCCGTGCT11.2987012987012987No Hit
ACATTATGATGTCGCCCGCGGCGATTCGGAAGGGTTTAAGGACACGTCTTAACAAATGGCATTACAGAATGGCTA11.2987012987012987No Hit
CTCTTCTACGGTTAATACTTTCCTCTTCGTCTTTTTCTACACCCTCGTTTAGTTGCTTCTTATTCCTTCCCGCTT11.2987012987012987No Hit
GTGATACGTCAAGAGCTCTTCATTGCAGATATCGTTTAGTTTCCCGTGGCACTTTACATCGTGTATAGAGATGGG11.2987012987012987No Hit
ATATACAACATTATGTAAAAGGAGTATACCAAAAATTCTCCTTCATAATATAGGAATTAACAAAATGGATCCGAT11.2987012987012987No Hit
ACTAACCCACGTCCAACTGCTGTTGACGTGGAACCTTTCCCCACTTCAGTCTTCAAAGTTCTCATGTGAATATTT11.2987012987012987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGAAG50.069.03
CAGTCCG50.069.019
GTGCAAT50.069.011
AGAAGTG50.069.05
ACAGAGG50.069.034
TACAGAG50.069.033
TGCAATT50.069.012
AATTCAG50.069.015
TTCAAAG50.069.049
TATGTTT50.069.044
GGACTAT50.069.040
GGTCTGG50.069.055
TCTGGAG50.069.057
CACAAGA50.069.01
GAAGTGT50.069.06
GTCCGAA50.069.021
AGAGGGA50.069.036
TGTTTCA50.069.046
GAGCTGC50.069.061
CAATTCA50.069.014