FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734059
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21120.12179516383237249No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACG1206.3808766E-520.12852530
GTATTAG8700.019.4321961
CCTATAC6300.019.1661553
TATACCG5050.019.1282025
TAGGTAG5200.018.5764265
GGTAGTC4900.018.3051057
ACATAGG5200.017.91711431
GTATAGA9300.017.8075161
TATTAGG9900.017.4237772
TAGTCAG5150.017.4165089
GGTAGGA5400.015.97179569
AGTCAGA6500.015.92219310
CGGACGG1808.075092E-515.33646131
GACATAG5950.015.07827130
CTATACT10050.014.7607984
TCTATAC9150.014.7045253
TTAGGTA9900.014.6359724
GAGTAAG6000.014.37627437
GTGAGCG1450.00521822514.2750699
ATTAGGT10700.014.1865373