FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4412653
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTG6950.019.8558169
TAGGTAG10850.013.9896155
GTATTAG19100.013.9080041
CTTACGC18450.013.83718569
CTAGGAC6550.013.6938243
CGCGTGT6100.013.57356269
GTATAGA19450.013.4803591
ACCTCGC3952.3283064E-913.1001728
GTATATA37550.012.954381
TATACTG26750.012.6381985
TTATACT28700.012.5006984
CTAGCCT7750.012.4634774
TCTATAC17800.012.4037413
CGATCTT53550.012.36955769
TACGCGT11350.012.15840268
TATACAG22750.011.8275855
TATTAGG21300.011.6613942
GATCTTA45400.011.62647269
CCGGCCC5056.1118044E-1011.61276113
TATACCG11650.011.5483925
Produced by FastQC (version 0.11.2)