FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452269
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCCA450.00462265530.66273711
TACGGCC500.007734875727.5964629
CACCGTG750.001992649622.9970517
TACTAGG800.002897038821.562122
TCTATAC2354.5656634E-1019.084773
TACACCG1108.2332717E-418.815775
CTTAGAC1200.001475078217.2496953
GTATTAG2151.2668297E-616.0515541
GTAATCC1300.00251883515.9227964
TATACAG3351.9463187E-1015.4457825
CCTAGAA1808.069297E-515.3330642
AGACAGT1851.0202503E-414.9170076
TAGGTAG1400.004129206814.7838195
TACACAG1400.004129206814.7838195
TATACGG2353.2113858E-614.6789695
CTACAGT1450.00520902314.275614
TAAGACT1709.10174E-414.2056314
GTCTATA1951.5906646E-414.1582951
TACAGTG1951.596655E-414.1520335
TTCTATA4202.7284841E-1113.964042
Produced by FastQC (version 0.11.2)