FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2670644
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37940.14206311286715864No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG11250.015.9482281
CATATAC16700.014.873483
TCTATAC11900.014.2051463
TATACGG8150.013.9683395
TATACAG15350.013.709135
GTATATA24400.013.1508781
GTGTATA10450.012.876861
GTGTAAG6450.012.8384681
CTATACT11750.012.6248784
TAGGACC5750.012.5993474
TATACTG17000.012.5814655
TAGACTG5551.8189894E-1212.4315565
TATTAGG12000.012.362092
GTATAGA12000.012.0761821
TCGGTCG2306.457723E-411.9991539
TACACAG7500.011.9591575
GTATTAT22950.011.7266391
GTGTAGG5351.2914825E-1011.6086141
ATATTAG17350.011.5342711
TGTACAC8450.011.431363
Produced by FastQC (version 0.11.2)