FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2744094
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33480.12200748225097244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC4200.018.8917453
ACCGCGC2601.7753337E-917.2483568
TACACCG5200.016.5849575
GTATAGG7700.014.786461
GCAGTGT11150.013.61404369
CACCGCG2851.6140766E-613.3147637
TATACCG6800.013.18991855
TCTAGGA8300.012.8847832
TAGGACT4651.546141E-1012.6119314
GATATGC30400.012.59675669
CTAATAC11250.012.5725943
CTAGACC7400.012.1206874
TATTAGG10000.012.0742882
CCTGGAC4601.7425918E-911.9992923
TAGACAG8100.011.9247895
CCGCGCA2902.818254E-511.8954179
CGATATG33100.011.77768168
CGTACAC4702.4429028E-911.7439893
TAGGTAG5005.16593E-1011.7288825
CGCGCAC2953.3566677E-511.693810
Produced by FastQC (version 0.11.2)