FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3294915
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51370.155906905033969No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC4500.014.56663869
TTACGTG12800.012.66794369
CTCCTAG3651.5411933E-712.288581
GACTCCT36300.012.07022469
TACACCG5107.185008E-1011.4991055
CGACTCC40350.011.45722769
GTATAAG12550.011.27174951
CTTACGT15950.011.03132669
GTATTAT23300.010.9578911
CCTTACG19550.010.941154569
GTATTAA15900.010.8498581
GGCCCGA2250.007468361410.73200910
CTATGCA9250.010.4419559
TATTATA33000.010.2449672
TACGTGT9450.010.22220269
CTAATAC14900.010.1871253
GTATTAG11600.010.1128161
GTATAAT19800.010.1068071
TAATACT21300.010.04147154
TATATAC26000.09.9511483
Produced by FastQC (version 0.11.2)