FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359403
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16420.12078831663605274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC3500.017.7410413
CGCACCT2359.484211E-917.61716366
TCTATAC6250.017.1102493
CTACACA4250.017.0453154
GTATTAG5550.016.7845341
CTGTGCG1704.966305E-516.2336339
CTATACT6200.016.1354454
TGAGCCG1300.002523751715.92144810
TATACGG5250.015.7698145
TCCGCAC2503.6684287E-715.18067964
CCGCACC2756.571463E-815.0552265
CTATGCA5500.015.0530049
GTCCTAG1851.0211417E-414.9195861
CTAAGGG3554.6929927E-1014.5759733
TAGACAG4353.6379788E-1214.2744015
TCGTGCG1450.005219076314.2744019
GTGTATG4600.014.2506381
GTCTAAG3901.2732926E-1014.1544791
GGCCTAT2202.8102171E-514.1142671
CTAATAC7100.014.0901063
Produced by FastQC (version 0.11.2)