FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849957
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9920.1167117865962631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC1751.4714351E-719.71450464
TGTAGAC3000.019.5490673
GGATAGG1258.728969E-519.3224871
CCGCACC1852.640718E-718.64885765
CGACGGG1301.1799342E-418.57822249
CGGGCGG1301.1799342E-418.57822252
GTATTAG3400.018.2670571
ACGGGCG1351.5754826E-417.8901451
TAGGCTA1200.001476821117.2491765
ACTATGC4050.017.0352218
ACTGCGT1653.8432016E-516.725498
GACCGTC1653.8432016E-516.725497
GTATATA7850.016.7027871
TCCTATA3106.002665E-1116.692752
CTAAGCA2552.5969712E-816.234524
TAGTCAG2351.8178253E-716.1472159
TAGGTAG2151.2748478E-616.0457465
CTTAGGG1300.002521789915.9223173
TCTAGGA1756.3560816E-515.7706752
TATAGCG2001.14177565E-515.5242595
Produced by FastQC (version 0.11.2)