FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2683419
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30740.1145553489782997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG12300.015.7077421
CGCACCT5000.015.17984466
CTTACAC6950.014.3949993
TCCGCAC4900.014.08148764
CCGCACC4950.013.9392565
GTATAGA12400.013.9116671
GTATATA28500.013.5582621
CTAGACT4609.094947E-1213.4993594
CTAATAC13100.013.430663
CTCCTAG3355.0304152E-813.3884211
TAGACTG6550.013.16706855
TAGGTAG6700.012.8722825
CCTAATA13150.012.8551422
TAGTCAG5950.012.7554599
ACTATGC10550.012.7527118
TCTATAC13050.012.6890533
TATACAG16800.012.115275
TTATACT19650.012.1139284
CTAGGAC4003.891182E-812.0744273
TAACCCG2306.4564904E-411.999434
Produced by FastQC (version 0.11.2)