FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2699694
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38870.14397928061476598No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT4250.023.54113269
ACGCGTG4950.019.51511468
GTATTAG11050.018.109571
TACACAG7750.017.3599725
GTATAGG8650.015.9546141
TAGGTAG6000.015.5238215
CGCGGGT2503.6704114E-715.18137837
TAGTCAG5100.014.8809469
TATTAGG11300.014.6542972
GGTAGTC6600.014.6349817
TATATAG15600.014.3739075
TATACAG13750.014.0498435
GTCTATA8000.013.8007411
TATACTG19500.013.7989525
TTATACT17350.013.71941954
TAGGACA7500.013.3389864
TACGCGT8700.013.08618369
GTATAGA12150.013.062431
GTATATA22850.012.985381
CGGGAGT2701.3517039E-512.77846450
Produced by FastQC (version 0.11.2)