FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2519479
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28770.11419027505289783No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG2901.261269E-714.2758221
GTATTAG10700.013.8644461
CTAATAC10550.013.7329233
GTATAAG9950.013.5225731
GCTTAGG7150.013.5104569
TAATACT18650.013.317414
GGCTTAG11100.012.12158769
CCTATAC6550.012.1130263
GTATAGA11500.011.9999651
GTATTAT18500.011.7486151
CCGCTCG2357.7389873E-411.7432489
GTATAGG7650.011.7254561
CCTATAG4451.3686076E-811.6277953
CGCACCT5056.0936145E-1011.61382966
ATACCGT10100.011.6122156
GTCTTAA7500.011.4999681
TATAAGA14500.011.4192972
TAGGTAG6151.0913936E-1111.217925
ACCGTCG5602.8740033E-1011.0879348
GTATATG14200.010.9330671
Produced by FastQC (version 0.11.2)