FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2443069
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27460.11239960885263577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG1855.456808E-616.782229
GTCCTAG2955.347829E-1016.3753781
GTATTAG9750.015.2177041
TATACTG13250.014.8413425
GTATATA17950.014.417241
GTCCTAA6550.013.6967451
TATTATA19200.013.4766862
CTAATAC11150.013.3050553
GTATAGA10450.013.2077561
TATACAG11350.013.0703385
CCTAGCG4002.7957867E-912.93973830
GTGTAAG5350.012.8991621
TATACCG6450.012.8370645
GTGTAGA9150.012.8216271
GTATTAT17550.012.7797261
CTGTGCG3255.056572E-712.7372749
TAATACT17400.012.4914944
TAGGTAG5800.012.4912395
CTATACT10000.012.42011454
ATAATAC18200.012.3215423
Produced by FastQC (version 0.11.2)