FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499131
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6380.12782215490522528No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC501.8598029E-434.50034764
GTGGCCC500.00773041127.60027948
CTGGGGA500.00773041127.6002794
GCTCGGA500.00773041127.6002794
CCGCACC658.6496904E-426.5387365
CGCACCT750.001992494622.99792966
TCGAGAC800.002896987621.5627254
GACCTCG800.002896987621.5627232
TCGGCTC800.002898672621.5605667
CTTACAC1451.2623497E-519.0346763
GAGCTAG950.00782794618.158085
ATTCGGG950.00783246918.1562612
CTAACCT1150.001108951818.0001834
CTATACT1602.944682E-517.2501754
TACGGTG1200.001475093517.2501757
TCTATAC2601.7607817E-917.2501753
ACTTGGG1503.5198618E-416.1001642
TATACTG3651.8189894E-1216.0686575
CTAATAC2654.1538442E-815.6228013
TCTAACC1400.004126206514.7858653
Produced by FastQC (version 0.11.2)