FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934922
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20780.10739450995957459No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT3700.023.30961466
CCGCACC4500.020.70054265
TCCGCAC4350.020.62176364
TAGGTAG4150.019.1175255
CTAATAC9350.018.4463443
ACTATGC8800.018.4232868
CTATGCA8000.018.1096979
GTATAGG5850.016.513681
GTATTAG8250.015.8917371
CCTAATA10000.015.5229992
TACTATG9150.015.4566257
TCACACA6300.015.33611330
ATCACAC6350.015.21457129
GTATATG11350.015.199031
TAATACT14850.015.0986754
ACCTAAT10550.014.3893741
GGTAGTC4353.6379788E-1214.2736547
ATCAGCC6050.014.25657254
GTATAGA9750.014.1545831
ACCTCGC1500.006537652613.7978658
Produced by FastQC (version 0.11.2)