FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1557251
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18340.11777163732757275No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCG2350.022.02491630
ACCTAGC2850.020.58036429
GTATAGA7850.020.2160031
GACTAGG2651.1641532E-1018.224168
GTATTAG5500.016.3086431
TATACTG10250.016.1540975
CTAGGCA3251.2369128E-1015.92110610
CACCTAG3101.0822987E-915.58171828
TAGGTAG3603.45608E-1115.3314355
TATAGAG6400.015.0923672
GCGACTC3453.0377123E-1015.00247934
GTATTAA6700.014.93242551
GTATAGG5800.014.8702821
TATACAG8450.014.6964055
AGACTAG3554.6929927E-1014.575667
TATACGG4850.014.2250445
CTAAGCA4152.3646862E-1114.1312354
TCTAAGC4152.3646862E-1114.1312353
CTAGACA4650.014.0954824
TAATACC7900.013.9734024
Produced by FastQC (version 0.11.2)