FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1633549
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18910.11576022512945738No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA3700.018.6493361
GTATATA10300.017.753081
TAGGACA3950.016.5935154
TCCTAAG3900.015.9226882
TCTATAC7000.015.7705613
GTATTAG5200.015.2601781
TAATACT8900.015.1166824
ATATTAA9450.014.9688041
TCTGGAC7200.014.3742083
TTATACT7700.014.3364344
CTATACA5550.014.2960744
TATATAC10650.013.9292372
CTAATAC5750.013.799243
GTGCGAT4001.9099389E-1013.79839611
TACACCG3304.1312887E-813.5901615
ATACAGT7950.013.452096
TAGGTAG3406.1079845E-813.1904525
ACAGTCC3406.110349E-813.1900478
GACCGAG2102.998585E-413.1417317
TACACAG5003.6379788E-1213.1092795
Produced by FastQC (version 0.11.2)