FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617523
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8180.13246470171961205No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCCT400.00259540934.510
TCCGCAC901.9926483E-730.66666664
CGCACCT951.0497415E-525.42105366
TAGACTG1101.1513348E-625.0909085
CCGCACC1251.2625424E-724.8465
ACAGTCC1056.018661E-419.7142878
GTAGGCC900.005731475519.1666663
TTCCGCA1903.4910045E-718.15789663
TAGGCCT1150.001109432818.04
TACACCG1150.001109432818.05
GGCAATC1250.001939241716.5618
CCCTTAT1503.5218277E-416.0999981
TCTATAC2804.7148205E-916.0178573
TACAGCG1554.5198982E-415.5806457
GTCTAAT2705.2335963E-815.3333331
GTGTTGC1605.7527155E-415.0937550
TATACAG4600.015.05
GTCCAGA1657.264793E-414.6363641
ACGGTGT1657.264793E-414.6363648
GTATTAG2851.0153235E-714.5263161
Produced by FastQC (version 0.11.2)