FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2706963
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG10750.013.7996971
GCTTAGG9500.012.71024669
CTTAGGT10550.012.42626769
GTATATA22600.012.3647731
GTATAGA10300.012.0579871
CTAATAC11800.011.9861393
ACCGCGC3601.7405018E-611.4988988
TATTATA28950.011.3203682
GTATAAT16550.011.2565521
TATACGG7800.011.0566335
GTCTATA8150.011.0058931
CCCATGC4401.4598118E-710.9762218
GTATTAT20250.010.9034641
GTATAGG8550.010.8944971
TAGGTGT9000.010.73309869
TAATACT19300.010.7243614
GTATAAG10500.010.5140551
CAGTGCG3301.0504625E-410.4535449
TATACTG14850.010.4535445
TTAGGAC5958.240022E-1010.4359753
Produced by FastQC (version 0.11.2)