FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2131333
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA9000.017.251011
TAATACT11650.015.9901024
TCTATAC7350.015.957873
CGCGCAT2603.306741E-815.9221440
TATACTG10900.015.8243865
GTATTAG6200.015.5815561
CTAATAC8000.015.5237243
CTTACAC4900.014.7844983
TTATACT8650.014.7560114
GTATATA11150.014.5434521
CCTAATA6900.014.4994892
TAGTACT6900.014.4988074
CTATAGT5850.014.1526824
TATAGGG5400.014.0550592
CTAGTAC6400.014.0144723
TAGGGGT3454.9112714E-913.9988494
GTCTAGT5950.013.916781
TTTATAC8250.013.7988653
ATACTGT11950.013.5682396
TATAGAG8950.013.4911422
Produced by FastQC (version 0.11.2)