FastQCFastQC Report
Thu 23 Mar 2017
HJNJTAFXX_n02_minidip01_38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNJTAFXX_n02_minidip01_38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3174859
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA10950.017.0163441
TAGACAG10600.013.66935355
TACACAG8850.013.6436115
TAGACTG5900.013.4487015
TCTATAC9800.013.0250733
TAGACCG9750.012.7380335
GTATTAG9700.012.4503891
CTAATAC10300.012.3927873
GACCGCG5054.7293724E-1112.2964247
CTTAGAC10950.012.2872593
TTAGACA12150.012.2094674
GTAGTAC8600.012.0344793
AGACCGC6600.012.0223246
CCGCGCG5501.6370905E-1111.9175949
CTTACAC6950.011.9132693
GTATTAT16200.011.7147841
TATAGAG11200.011.7051492
GTCTATA6201.8189894E-1211.6873011
GTCTAGA9300.011.5017881
CCTAATA8700.011.4997952
Produced by FastQC (version 0.11.2)